We recommend maintaining the pBirAcm with chloramphenicol (10 ug/ml) until the overnight is diluted to get the cell culture growing exponentially prior to induction.
Cells are supplied as 10 vials of 100ul liquid culture in 30% glycerol. Store at -80°C.
Note: Recombinant proteins made as insoluble inclusion bodies in the BL21DE3 host strain are not biotinylated efficiently even with the BirA over-production provided by the pBirAcm vector.提供全套的蛋白、肽生物素化的产品,其主要产品包括:生物素蛋白连接酶(BirA);生物素化菌株;AviTag 载体;生物素化重组蛋白.
Avidity, LLC was founded in 1996 by Millard Cull, Larry Lansing, Dr. Ron Gill, and Dr. Mark Seville to develop peptide tags and molecular biology products based upon the extraordinary affinity of biotin for avidin or streptavidin. While working at Affymax as a researcher, Millard was involved in the discovery of the AviTag technology, saw its commercial potential, and obtained the exclusive rights from Affymax. He started Avidity as a reagent company that sublicenses the right to use the AviTag technology to other companies and sells products for use with the technology.