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主营:酶,缓冲剂,酰,生物素克隆和表达系统,AviTag,蛋白质的纯化和鉴定
℡ 4000-520-616
℡ 4000-520-616
Avidity/Bulk BirA-RT - Bulk BirA-RT: BirA biotin-protein ligase bulk lyophilized reaction kit/1/Bulk BirA-RT
产品编号:BulkBirA-RT
市  场 价:¥19600.00
场      地:美国(厂家直采)
产品分类: 蛋白类>多肽>多肽合成>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$980.00
品      牌: Avidity
公      司:Avidity
公司分类:
Avidity/Bulk BirA-RT - Bulk BirA-RT: BirA biotin-protein ligase bulk lyophilized reaction kit/1/Bulk BirA-RT
商品介绍

Product Information

Kit supplied as:

  • BirA Ten (10) vials of 30µg each 3mg/mL lyophilized BirA biotin-protein ligase (300ug total)
  • Storage Buffer One (1) bottle of 500uL BirA Resuspension buffer
  • SuperMix One (1) tube; 10mL of 10x SuperMix buffer
  • ATP One (1) tube; 550 mg of ATP under argon gas
  • Additional tubes 10 labeled 2mL tubes for SuperMix/ATP storage

The BirA biotin-protein ligase (EC 6.3.4.15) adds d-biotin covalently to biotin-acceptor peptides/proteins via an ATP intermediate (biotinyl 5"-adenylate) in a highly efficient and targeted manner. The downstream applications of enzymatically biotinylated proteins are varied, important and powerful. The well known biotin-avidin/streptavidin interaction is often exploited for affinity chromatography or protein immobilization on surfaces or substrates. Protein detection via anti-biotin antibodies or avidin/streptavidin-reporter enzyme conjugates (-HRP, -alkaline phosphatase) or fluorescent probes becomes possible. Multimeric forms of biotinylated MHC molecules are popular tools in immunobiology.

When used in combination with our AviTag™ biotin-acceptor peptide amino acid sequence, biotinylation occurs at twice the rate of the natural E. coli BCCP substrate and as much as an order of magnitude or more over other Biotinylation of Peptide sequences. The AviTag™ sequence consequently requires less of the BirA enzyme and shorter incubation times to biotinylate to completion than do other sequences available. If protein instability or protease activities are a concern this may be important to downstream success.

Our BirA enzyme is E. coli wild-type, encoded by the birA gene, and purified to greater than 99% purity by traditional methods.

Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase; biotin-[acetyl-CoA-carboxylase] ligase; biotin-[acetyl-CoA carboxylase] synthetase; acetyl CoA holocarboxylase synthetase; acetyl CoA holocarboxylase synthetase; biotin:apocarboxylase ligase; biotin holoenzyme synthetase; HCS.

品牌介绍

提供全套的蛋白、肽生物素化的产品,其主要产品包括:生物素蛋白连接酶(BirA);生物素化菌株;AviTag 载体;生物素化重组蛋白.

Avidity, LLC was founded in 1996 by Millard Cull, Larry Lansing, Dr. Ron Gill, and Dr. Mark Seville to develop peptide tags and molecular biology products based upon the extraordinary affinity of biotin for avidin or streptavidin. While working at Affymax as a researcher, Millard was involved in the discovery of the AviTag technology, saw its commercial potential, and obtained the exclusive rights from Affymax. He started Avidity as a reagent company that sublicenses the right to use the AviTag technology to other companies and sells products for use with the technology.

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